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XIAP plays an important role in the synergistic cytotoxicity of PZQ and PTX.

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posted on 12.12.2012 by Zhen Hua Wu, Ming-ke Lu, Long Yu Hu, Xiaotong Li

(A) Western blot showed XIAP expression in DLD-1 cells at 24 h after transfection with pcDNA3-myc-XIAP or control vector. (B) 24 h after transfection with pcDNA3-myc-XIAP and control vector, DLD-1 cells were treated with 20 µM PZQ alone, 10 nM PTX alone or the combination for 48 h, and then cell viability was determined by MTT assay. (C) 24 h after transfection with pcDNA3-myc-XIAP and control vector, DLD-1 cells were treated with a combination of 20 µM PZQ and 10 nM PTX. Then the sub-G1 fraction was determined by flow cytometry. (D) DLD-1 cells were infected with a lentivirus encoding control shRNA or XIAP shRNA for 48 h, and expression of XIAP was analyzed by Western blot. (E) DLD-1 cells were infected with a lentivirus encoding control shRNA or XIAP shRNA for 48 h, and then treated with 20 µM PZQ alone, 10 nM PTX alone or the combination for 48 h. The cell viability was determined by MTT assay. All Values are shown as mean±SEM from three separate experiments.

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