Widefield FPALM Enables Penetration Into Sample.

Schematic of experimental setup. A) Diagram demonstrating how Casper fms embryos were injected at the 1-cell stage with linearized DNA plus phenol red indicator. B) A widefield image of a 24 hpf zebrafish embryo. Outlined in red is the region of the tail that FPALM images were acquired at, as it is the thinnest part of the fish and also has minimum autofluorescence. C) A 24 hpf embryo was placed in a microscope slide with a single shallow depression, immersed in a drop of non-lethal tricaine (solubilized in egg water). A cover slide (#1.5) was placed over the slide, covering the embryo and the coverslip was mounted on a microscopy setup suitable for FPALM.