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Western blotting analyses of Tie2 and VEGFR2 in cultured glomerular endothelial cells.

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posted on 05.04.2013, 01:43 by Bao-hai Zhang, Wenying Wang, Hongxia Wang, Jiming Yin, Xiang-jun Zeng

A: The images are derived from identical gels of Tie2 and GAPDH, and each lane was loaded with the same amount of protein (50 µg ). The numbers indicate the expected molecular weights of the bands. B: Densitometry of Tie2 levels normalized to GAPDH for each treatment condition in A. The data are expressed as the means±SD (n = 3, *p<0.05 vs. control group ). C: Images are derived from identical gels of VEGFR2 and GAPDH, and each lane was loaded with the same amount of the protein (50 µg ). D: Densitometry of VEGFR2 levels normalized to GAPDH for each treatment condition in C. The data are expressed as the means±SD (n = 3, *p<0.05 vs. control group).

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