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Western blot results of deer exposed to body secretions and excreta from CWD+ deer.

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posted on 21.02.2013, 05:39 by Candace K. Mathiason, Sheila A. Hays, Jenny Powers, Jeanette Hayes-Klug, Julia Langenberg, Sallie J. Dahmes, David A. Osborn, Karl V. Miller, Robert J. Warren, Gary L. Mason, Edward A. Hoover

Western blot demonstration of the typical PK digestion band shift (28–35 kD) associated with prion infection in the brain (medulla oblongata at obex) of deer receiving blood, brain, saliva or environmental exposure, but not urine and feces from CWD-infected donors. Lanes 1–4 represent CWD+/− deer controls (10% brain homogenate of medulla at obex) without and with PK digestion at 25 µg/ml. Lanes 5–12, 10% brain homogenate of blood, brain, urine/feces or saliva inoculated deer without and with PK digestion at 25 µg/ml. Lanes 13–15, brain homogenate from deer environmentally exposed to CWD+ fomites without and with PK digestion at 25 and 50 µg/ml.

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