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Western blot probed with an anti-YFP antibodies.

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posted on 05.11.2014 by Matthias T. Buhmann, Nicole Poulsen, Jennifer Klemm, Matthew R. Kennedy, C. David Sherrill, Nils Kröger

The extracts obtained from identical amounts of either wild type (WT) or AC3362-YFP bearing cell walls by treatment with a hot SDS solution (SDS) or with an ammonium fluoride solution at pH 4–5 (AF) were loaded. In lane ‘ctrl.’ 100 ng recombinant Sil3-GFP (isolated from E. coli) was loaded.

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