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Western blot analysis of cystatin C in the CSF of patients suffering from sCJD.

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posted on 2008-12-05, 01:26 authored by Gino Miele, Harald Seeger, Denis Marino, Ralf Eberhard, Mathias Heikenwalder, Katharina Stoeck, Max Basagni, Richard Knight, Alison Green, Francesca Chianini, Rudolf P. Wüthrich, Christoph Hock, Inga Zerr, Adriano Aguzzi

Equivalent amounts of CSF protein (10 µg) from late-stage sCJD patients and from appropriate age-matched non-sCJD patients were fractionated under non-reducing SDS-PAGE conditions and analyzed by Western blot using a polyclonal anti-human cystatin C antibody. No difference was found in CSF cystatin C levels of sCJD patients vs. controls.

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