WT Decision Tree
The flowchart of scheme for prediction of effects of Pro mutations on protein stability derived from structural analysis of WT-CcdB crystal structure alone. A and I refer to active and inactive predictions, respectively. The numbers at each branch indicate the number of active and inactive mutants satisfying the “Yes” or “No” criteria of the respective branch. The numbers at each A and I prediction bubble correspond to the number of CcdB Pro mutants ending at that bubble. The numbers of misclassified mutants are shown in italics.