Validation of significantly up-regulated SuperSAGE Tags annotated to N. attenuata (A) and R. irregularis (B) by qPCR.
Relative transcript levels of selected plant (A) and fungal (B) Tags significantly up-regulated (P < 0.05) after the infection of Nicotiana attenuata by Rhizophagus irregularis. qPCR analysis was carried out on root samples harvested 35 days after inoculation. Elongation factor-1 alpha was used as a reference gene for normalization. Transcript levels were analyzed in four biological replicates ((+ se, N = 4).