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Validation of significantly up-regulated SuperSAGE Tags annotated to N. attenuata (A) and R. irregularis (B) by qPCR.

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posted on 2015-08-20, 03:51 authored by Karin Groten, Nabin T. Pahari, Shuqing Xu, Maja Miloradovic van Doorn, Ian T. Baldwin

Relative transcript levels of selected plant (A) and fungal (B) Tags significantly up-regulated (P < 0.05) after the infection of Nicotiana attenuata by Rhizophagus irregularis. qPCR analysis was carried out on root samples harvested 35 days after inoculation. Elongation factor-1 alpha was used as a reference gene for normalization. Transcript levels were analyzed in four biological replicates ((+ se, N = 4).