Validation of medulloblastoma DHS sites in a cohort of OTX2-expressing and -nonexpressing cells.
Nuclei of medulloblastoma cell lines were treated with increasing concentrations of DNase (indicated as Units per reaction), and then the relative proportion of DNA remaining at the indicated regions were determined by qPCR. Open and solid markers indicate OTX2-expressing and -nonexpressing cell lines, respectively. See Fig. S2 for detailed graphs.