Upregulation of TRPV4 mRNA and protein in the liver tissues from liver fibrosis patients and CCl4-treated rats.

A. The level of the TRPV4 was analyzed by immunohistochemistry in human normal liver and liver fibrosis patients. Representative views from each group are presented (original magnification, ×50). B. Liver tissues from liver fibrosis patients tissue were double stained with TRPV4 and α-SMA antibodies. Representative photomicrographs are shown (original magnification, ×40) in B. C. Pathology observation of the experimental rat liver sections stained with hematoxylin and eosin (H&E) staining and massion staining (×200). D. The level of the α-SMA was analyzed by immunohistochemistry in vehicle control livers and liver fibrotic tissue. Representative views from each group are presented (original magnification, ×50). E-F. Total RNAs were isolated from the livers of CCl₄-treated rats or vehicle-treated groups. The expression of TRPV4 mRNA was assessed by RT-PCR(E) and realtime PCR(F). Representative images of three independent experiments are shown. *p<0.05, **p<0.01 vs. vehicle control. G. Whole-cell extracts were isolated from the liver tissues of CCl₄-treated rats or vehicle-treated groups, and subjected to immunoblot for TRPV4 and β-actin control. Representative images of three independent experiments are shown. *p<0.05, **p<0.01 vs. vehicle control.