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Upon infection with T. gondii, 3d mice display a significant number of T cells expressing activation markers in the surface.

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posted on 2010-08-26, 01:09 authored by Mariane B. Melo, Pia Kasperkovitz, Anna Cerny, Stephanie Könen-Waisman, Evelyn A. Kurt-Jones, Egil Lien, Bruce Beutler, Jonathan C. Howard, Douglas T. Golenbock, Ricardo T. Gazzinelli

(A) Flow cytometry analysis of splenocytes isolated from non-infected animals or at 8 days after infection with T. gondii. Cells were stained with fluorescent antibodies anti-CD3, anti-CD4, anti-CD8, anti-CD25, anti-CD69 and anti-CD154. (B–C) IFNγ-producing CD4+ and CD8+-T cells present in splenocytes of C57BL/6 and 3d mice non-infected or at 8 days post-infection, as measured by flow cytometry. A panel with results for IFNγ -positive CD8+-T cells from representative individual wild-type and 3d mice is shown in B. One asterisk indicates that p<0.05 when comparing results from 3d or MyD88-deficient mice to WT animals infected with T. gondii. Two asterisks indicate that p<0.05 when comparing the results from 3d mice with MyD88-deficient animals infected with T. gondii. Data are representative of experiments done two times, using four mice per group (error bars, s.e.m.).