Up-regulation of NHE1 protein expression in reactive hippocampal astrocytes.
A. Expression of NHE1 protein in hippocampal astrocytes. Immunofluorescence signals of NHE1 and GFAP were shown under normoxia control or OGD (2 h) plus 0 h, 5 h, or 24 h REOX. Green: GFAP, red: NHE1 (arrows), blue: To-pro-3 nuclear staining. Scale Bar: 20 µm. B, C. Quantification of GFAP (B) and NHE1 protein (C) expression with immunoblotting. Hippocampal astrocytes were subjected to 2 h OGD followed by 5 or 24 h REOX. Total pixel density in each protein band (minus background) was calculated using Image J. Data are expressed as the ratio of either GFAP or NHE1 to the corresponding α-tubulin band and normalized to the control. Data are mean ± SEM (n = 4). *p<0.05 vs. normoxia.