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Unfolding kinetic constant derived from limited proteolysis of HLL.

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posted on 18.01.2016, 15:12 by Qiang Wang, Nicklas Waterhouse, Olusegun Feyijinmi, Matthew J. Dominguez, Lisa M. Martinez, Zoey Sharp, Rachel Service, Jameson R. Bothe, Elliott J. Stollar

A range of concentrations of trypsin (A) and thermolysin (B) are used and degradation is followed by tryptophan fluorescence emission at 330 nm. The fits are to Eq 5 as indicated in the methods.

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