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UVR suppresses asthma-like Th2 responses to a similar degree in female and male (initially) vitamin D3-deficient mice.

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posted on 2013-02-19, 22:40 authored by Shelley Gorman, Naomi M. Scott, Daryl H. W. Tan, Clare E. Weeden, Robert C. Tuckey, Jacqueline L. Bisley, Michele A. Grimbaldeston, Prue H. Hart

Vitamin D3-deficient female or male BALB/c mice were UV irradiated (8 kJ/m2) and three days later were sensitized and boosted with OVA and alum on days 0 and 14, respectively. On days 21, 22 and 23 mice were challenged with an OVA aerosol. After a further 24 h, bronchoalveolar lavage fluid was obtained. In (A), eosinophil numbers, and in (B), IL-5 concentrations in the bronchoalveolar lavage fluid are shown. In (C) and (D), eosinophil numbers and IL-5 concentrations were normalized relative to eosinophils numbers and IL-5 concentrations measured for non-irradiated OVA-sensitized, -boosted and -challenged mice to remove the effects of gender on allergic sensitization. The mean result from each experiment was used to calculate the results shown (mean + SEM, n = 3 experiments, *p<0.05, n = 4 mice/group/experiment), with results from female and male mice depicted as open and closed bars, respectively.