UVR suppresses asthma-like Th2 responses to a similar degree in female and male (initially) vitamin D3-deficient mice.
Vitamin D3-deficient female or male BALB/c mice were UV irradiated (8 kJ/m2) and three days later were sensitized and boosted with OVA and alum on days 0 and 14, respectively. On days 21, 22 and 23 mice were challenged with an OVA aerosol. After a further 24 h, bronchoalveolar lavage fluid was obtained. In (A), eosinophil numbers, and in (B), IL-5 concentrations in the bronchoalveolar lavage fluid are shown. In (C) and (D), eosinophil numbers and IL-5 concentrations were normalized relative to eosinophils numbers and IL-5 concentrations measured for non-irradiated OVA-sensitized, -boosted and -challenged mice to remove the effects of gender on allergic sensitization. The mean result from each experiment was used to calculate the results shown (mean + SEM, n = 3 experiments, *p<0.05, n = 4 mice/group/experiment), with results from female and male mice depicted as open and closed bars, respectively.