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UPS perturbation induces similar gene expression responses to N. parisii infection.

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posted on 19.06.2014, 15:15 by Malina A. Bakowski, Christopher A. Desjardins, Margery G. Smelkinson, Tiffany A. Dunbar, Isaac F. Lopez-Moyado, Scott A. Rifkin, Christina A. Cuomo, Emily R. Troemel

A) Expression of C17H1.6p::gfp and F26F2.1p::gfp in the intestine (pharyngeal myo-2p::mCherry expression is a marker for the presence of the transgene) following infection with N. parisii (8 and 24 hpi). Scale bars  = 100 µm. B) Expression of C17H1.6p::gfp and F26F2.1p::gfp following RNAi against ubq-1, ubq-2, pas-5 and rpn-2 in the absence of infection. C) Expression of endogenous mRNA of C17H1.6 and F26F2.1, as well as the SCF ligase components skr-1, skr-3, skr-4, skr-5 and cul-6 following RNAi against ubq-1, ubq-2, pas-5 and rpn-2 in the absence of infection, as assessed by qRT-PCR. Due to the very large changes in expression of C17H1.6 and F26F2.1 genes, these are presented on a separate graph to allow for expansion of the y-axis and easier observation of expression changes in SCF ligase components. Mean +/- SEM of two to three independent experiments.

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