U87 localization to the peri-endothelial space is blocked by the CXCR4 antagonist AMD 3100.

(A) GFP-expressing U87 cells (green) are localized to mCherry-expressing HBMECs (red). (B) Treatment of parallel co-cultures as in A with AMD 3100 results in failure of U87 cells (green) to make consistent contact with endothelial cells (red). Scale bar equals 25 microns. (C) The CXCR4 antagonist AMD 3100 redistributes U87 cells, decreasing the number of cells within the nearest proximity to HBMECs and increasing the number of cells at greater distance. * = p<0.05 as determined by two-way ANOVA. (D) AMD 3100 increased the mean distance between U87 cells and HBMECs but had no effect on the mean distance between U87 cells and HUVECs. * = p<0.05 as determined by two-tailed t-test.