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Triple staining of a pupal wing tissue.

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posted on 24.06.2015, 04:18 by Yoshikazu Ohno, Joji M. Otaki

The tissue was stained with Hoechst 33342 for nuclei, BODIPY FL Thapsigargin for ER, and MitoTracker Orange for mitochondria. White arrows indicate endosome-like or autophagosome-like unstained structures. (a) Low magnification view. (b) High magnification view. Original scanning images were acquired with 1.0 μm intervals, and those 23 images were stacked to produce the final image of (b) and (d). (c) Hoechst 33342 staining. A part of the image shown in (a) was enlarged and its contrast was enhanced. Right and left images are identical except that nuclei with possible circular arrangements are highlighted in white. (d) 3D image of an epithelial tissue.