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Treg/Th17 balance regulated liver fibrosis progression in mouse models

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posted on 20.06.2012 by Jing Li, Shuang-Jian Qiu, Wei-Min She, Fu-Ping Wang, Hong Gao, Lei Li, Chuan-Tao Tu, Ji-Yao Wang, Xi-Zhong Shen, Wei Jiang

. (A) Experimental protocol: Mice were i.v. injected with ConA weekly for up to four weeks to establish liver fibrosis models. Depletion of CD25+ or IL-17+ cells during fibrosis progression was performed with i.p. injection of either anti-CD25 or anti-IL-17 mAb one day before and after ConA injection twice a week. (B) Representative dot plots of CD4+CD25+ or CD4+IL-17+ cells in mouse livers are shown at week 4 after the administration of anti-CD25 or anti-IL-17 or isotype control mAb. (C) The frequencies of CD4+CD25+ cells in mouse livers and spleens with administration of anti-CD25 or isotype control mAb were determined at week 0, 1, 2, 3 and 4 after ConA injection. ***, p<0.001 compared to isotype at week 0. (D) The frequencies of CD4+IL-17+ cells in mouse livers and spleens with administration of anti-IL-17 or isotype control mAb were determined at week 0, 1, 2, 3 and 4 after ConA injection. **, p<0.01; ***, p<0.001 compared to isotype at week 0. (E) Serum ALT levels at week 0, 1, 2, 3 and 4 after ConA injection. (F) Representative Masson staining of livers in ConA or PBS treated mice is shown at week 4 after the administration of anti-CD25 or anti-IL-17 or isotype Ab (× 100 and × 200 magnification). n = 6–8 per group. (G) The mRNA levels of α-SMA, PDGF-BB, TGF-β, collagen type I and III in mouse livers at week 4 after ConA injection are shown in four groups: isotype, isotype + ConA, anti-CD25+ ConA and anti-IL-17+ ConA groups. Data are expressed as the mean ± SEM; *, p<0.05; **, p<0.01; ***, p<0.001.

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