Translocation of GFP-tagged RasGRP1 in living cells after treatment with PMA or I3A.
Cells expressing GFP-RasGRP1 were treated with 1000 nM PMA or I3A. The translocation pattern was examined in live cells as a function of time. The results of duplicate experiments with I3A are shown. The images shown are representative of four independent experiments. The scale bar represents 10 µm.