Transient transfection-reporter assay of the effect of RXRγ on <i>Glut1</i> promoter.
figure
posted on 2011-05-31, 00:09 authored by Satoshi Sugita, Yasutomi Kamei, Fumiko Akaike, Takayoshi Suganami, Sayaka Kanai, Maki Hattori, Yasuko Manabe, Nobuharu Fujii, Takako Takai-Igarashi, Miki Tadaishi, Jun-Ichiro Oka, Hiroyuki Aburatani, Tetsuya Yamada, Hideki Katagiri, Saori Kakehi, Yoshifumi Tamura, Hideo Kubo, Kenichi Nishida, Shinji Miura, Osamu Ezaki, Yoshihiro Ogawa<p>(<i>A</i>) <i>Glut1</i>-Luc plasmid, with or without
RXRγ and/or PPARδ expression vectors, was transfected into the
quadriceps muscle of C57BL6 mice. Activation of the luciferase reporter
gene was measured in relative light units and normalized to dual
luciferase activity. Mean values from experiments
(n = 5) are shown as fold induction, where the
activity in the absence of RXRγ is the reference value (set at 100).
(<i>B</i>) Schematic representations of serial deletion of
<i>Glut1</i> promoter constructs are shown in the figure.
Squares denote the putative PPAR/RXR binding sites. Open bars;
<i>Glut1</i>-Luc without RXRγ and PPARδ expression
vectors, and filled bars; <i>Glut1</i>-Luc with RXRγ and
PPARδ expression vectors. The activity in the absence of RXRγ
and PPARδ in each experiment for different
<i>Glut1</i>-Luc construct in the reference value (set at
100). ** <i>P</i><0.01, compared with the value of
wild-type promoter in the absence of RXRγ/PPARδ.</p>
