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Transgene integration and expression analysis in three cloned lambs.

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posted on 2013-03-21, 04:53 authored by Shengwei Hu, Wei Ni, Wujiafu Sai, Ha Zi, Jun Qiao, Pengyang Wang, Jinliang Sheng, Chuangfu Chen

PCR analysis using primers specific for the shRNA expression cassette (A) and Neo (B). (C) Expression of the shRNA targeting MSTN in muscle tissues of transgenic sheep M17, M18 and M23. Negative control (NC): control sheep; positive control (PC): ploxP-shMSTN3 vector. M: Maker; TF-s2: Transgenic cell clone TF-s2; TF-s19: Transgenic cell clone TF-s19. (D) Western blot analysis of MSTN protein expression in muscle tissues of transgenic sheep M17, M18 and M23 and control sheep (Ctr1, Ctr2 and Ctr3).

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