Transgene integration and expression analysis in three cloned lambs.
PCR analysis using primers specific for the shRNA expression cassette (A) and Neo (B). (C) Expression of the shRNA targeting MSTN in muscle tissues of transgenic sheep M17, M18 and M23. Negative control (NC): control sheep; positive control (PC): ploxP-shMSTN3 vector. M: Maker; TF-s2: Transgenic cell clone TF-s2; TF-s19: Transgenic cell clone TF-s19. (D) Western blot analysis of MSTN protein expression in muscle tissues of transgenic sheep M17, M18 and M23 and control sheep (Ctr1, Ctr2 and Ctr3).