Transcriptional response of components of the Cu efflux network and phenotypic consequences of their deletion.

<p>Temporal changes in mRNA levels in response to a step increase in Cu to growth sub-inhibitory level (0.85 mM) reveals pulsed responses in transcript expression of the efflux pump <i>yvgX</i> and the two metallochaperones <i>VNG0702H</i> and <i>VNG2581H</i>. Expression of the metalloregulator <i>VNG1179C</i> does not change, suggesting that it is post-transcriptionally activated. Error bars are s.e.m for n = 5. Points (X) within each expression profile were normalized to the expression value at t = 0 (X₀). This ratio is plotted using a Log₁₀ transformation to accommodate large differences in dynamic range. Negative values indicate decreases in expression relative to expression at t = 0.</p>