Toxicity and anti-vector cellular immune responses.

Toxicity and the induction of anti-vector cellular immune responses were measured. Liver toxicity is expressed as a measured of ALT expression 48 hours after i.m. (A) and i.v. (B) injection. Anti-adenovirus cellular responses were measured 4 wks after i.m. (C) and i.v. (D) injections. Naïve BALB/c splenocytes infected with wild-type Ad5 were used as antigen presenting cells and were co-cultured with splenocyted from immunized mice. Anti-Ad5 cellular responses were measured by ELISPOT. Data represent groups of 5 mice and error bars indicate standard error. The data were analyzed by one-way ANOVA (* = p<0.05 and *** = p<0.001).