Topoisomerase II knockdown has a greater effect on G2 arrest induced by etoposide than by voreloxin or doxorubicin.
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A, A549 cells were transfected with siRNA targeting topoisomerase IIα (Topo IIα KD), or with scrambled control siRNA for 48 h, when a sample was taken to confirm the knockdown by Western blot. Blank = no siRNA. At 48 h, cells were treated for 16 h with a dose-titration (0.001–3 µM) of voreloxin and stained with BrdU followed by flow cytometry analysis. The percentages of cells in the G2 phase of the cell cycle, calculated from these data, are represented by the line graphs. Data are representative of 6 independent experiments. B, Cells were transfected and treated as described in (A) with a dose-titration (0.001–3 µM) of voreloxin, doxorubicin or etoposide. The same total transfected cell population was split and seeded for treatment with each drug. The percentages of cells in the G2 phase of the cell cycle are represented by the line graphs. Histograms are shown in Figure S3a, S3b, S3c. Data were consistent in 2 independent experiments.