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Tissue-specific expression of giant and alpha isoforms of nesprins.

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posted on 09.04.2014 by Nguyen Thuy Duong, Glenn E. Morris, Le Thanh Lam, Qiuping Zhang, Caroline A. Sewry, Catherine M. Shanahan, Ian Holt

Quantitative PCR to show mRNA expression of nesprin isoforms relative to the expression of two endogenous house-keeping controls. Charts represent the mean relative expression ± SEM, measured in cDNA preparations from 20 human tissues and 7 cell lines. These values are given in Tables S3 and S4 in File S1. Bar charts show relative expression of (A) nesprin-1-giant and nesprin-2-giant, and (B) nesprin-1-alpha-2 and nesprin-2-alpha-1.