Tissue-specific expression of giant and alpha isoforms of nesprins.
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posted on 2014-04-09, 03:41 authored by Nguyen Thuy Duong, Glenn E. Morris, Le Thanh Lam, Qiuping Zhang, Caroline A. Sewry, Catherine M. Shanahan, Ian HoltQuantitative PCR to show mRNA expression of nesprin isoforms relative to the expression of two endogenous house-keeping controls. Charts represent the mean relative expression ± SEM, measured in cDNA preparations from 20 human tissues and 7 cell lines. These values are given in Tables S3 and S4 in File S1. Bar charts show relative expression of (A) nesprin-1-giant and nesprin-2-giant, and (B) nesprin-1-alpha-2 and nesprin-2-alpha-1.
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cell biologyCellular structures and organellesCell membranesMembrane proteinsTransmembrane proteinsMolecular cell biologyBiochemistrydnaDNA amplificationproteinsStructural proteinsrnaRNA synthesisNucleic acidsimmunologyimmunochemistrygeneticsgene expressionGene regulationGenetic dominanceAutosomal dominant traitsHuman geneticsMolecular geneticscardiologyCardiovascular diseasescardiomyopathiesneurologyMuscular dystrophiesalphaisoforms
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