Timecourse of glucose- and K+-induced insulin release from isolated islets.
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(A) Isolated wt (black) and CgB-ko (white) islets were incubated in the presence of 1 mM (circles) or 20 mM (squares) glucose. Insulin in the medium was measured at 2 min intervals during 20 min after adjusting the glucose concentration; n = 4. (B) Same data as in A, but plotted as release per 2 min. (C) Insulin secretion from wt islets (black) and CgB-ko (white) islets was measured after 15 min incubation in the following conditions: 1 mM glucose+5 mM K+ (1G), 20 mM glucose+5 mM K+ (20 G) and 1 mM glucose+50 mM K+ (50 K+); n = 10. (D) Proinsulin content of wt (black) and CgB-ko islets (white); (n = 8 each). (E) Proinsulin release during 30 min batch incubations in buffer containing 20 mM glucose. *P<0.05; **P<0.01; ***P<0.001.