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Timecourse of glucose- and K+-induced insulin release from isolated islets.

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posted on 21.02.2013 by Stefanie Obermüller, Federico Calegari, Angus King, Anders Lindqvist, Ingmar Lundquist, Albert Salehi, Maura Francolini, Patrizia Rosa, Patrik Rorsman, Wieland B. Huttner, Sebastian Barg

(A) Isolated wt (black) and CgB-ko (white) islets were incubated in the presence of 1 mM (circles) or 20 mM (squares) glucose. Insulin in the medium was measured at 2 min intervals during 20 min after adjusting the glucose concentration; n = 4. (B) Same data as in A, but plotted as release per 2 min. (C) Insulin secretion from wt islets (black) and CgB-ko (white) islets was measured after 15 min incubation in the following conditions: 1 mM glucose+5 mM K+ (1G), 20 mM glucose+5 mM K+ (20 G) and 1 mM glucose+50 mM K+ (50 K+); n =  10. (D) Proinsulin content of wt (black) and CgB-ko islets (white); (n = 8 each). (E) Proinsulin release during 30 min batch incubations in buffer containing 20 mM glucose. *P<0.05; **P<0.01; ***P<0.001.

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