Time-lapse imaging for microglial phagocytosis of injured cells after NMDA treatment.
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Time-lapse images (at 20-min intervals) of EGFP-expressing microglia (green) and PI-positive injured neurons (red) in the pyramidal cell layer. White arrowhead and yellow arrow indicate the engulfing microglial cell and engulfed injured neuron, respectively. PI fluorescence of the injured neuron disappeared between 46 h 00 min and 46 h 20 min after the beginning of time-lapse imaging. Scale bars = 20 µm.