Time-kill kinetics of NaCl against P.aeruginosa Pa1 according to bacterial phase of growth and incubation temperature.

Inoculum in stationary phase of growth (A) and in logarithmic phase of growth (B) for Pa1 (NaCl MBC: 7%). Bacterial suspension in NaCl at the MBC value were incubated at 37°C (green dotted line, open circles) or 4°C (red dotted line, filled circles). Controls (full lines) were not exposed to NaCl and were incubated at 37°C (green line, open circles) or 4°C (red line, filled circles). The horizontal dotted line indicates a 4-log reduction in viability. B: dotted green (open circles) and red (filled circles) lines are superposed.