Time courses of and correlations between HIV-DNA and HIV-RNA measurements.
Figure 2 depicts time courses of and correlations between HIV-DNA and HIV-RNA measurements obtained before (panels A, B, C), during (panels D, E) and after early antiretroviral therapy initiation (panels F, G, H) in primary HIV-infected individuals. Panels A, B and C show the time dependency of pre-treatment HIV-DNA and -RNA measurements (n = 67 patients). The curves show fits from linear regression including linear and quadratic terms. Final model selection was based on adjusted R2 values. Panel A plots plasma HIV-RNA (log10 copies/mL) against time since infection, panel B shows Cell associated HIV-DNA (log10 copies/million cells), and panel C displays the correlation analysis of pre-treatment HIV-RNA and HIV-DNA. Panels D and E depict the dynamics of HIV-RNA (n = 700 measurements) and HIV-DNA (n = 737 measurements) during treatment with combination antiretroviral therapy in 59 patients. Panel D shows longitudinal HIV-RNA measurements during treatment. Note that undetectable HIV-RNA measurements were artificially set to 0. Panel E shows results from non-linear modeling analyses of the longitudinal course of HIV-DNA under antiretroviral therapy. Panels F and G show the evolution of viral marker pairs (HIV-RNA and HIV-DNA; n = 203 measurements) up to 18 months after therapy cessation (n = 33 patients). Curves were fitted with linear mixed models with random intercepts and random slopes. Panel H displays the correlation between post-therapy HIV-RNA and HIV-DNA measurements. The correlation coefficient shown is the median of 33 individual Spearman correlation analyses for each patient.