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Time course of GOS formation (A) and formation and degradation of GOS during lactose conversion (B) catalyzed by B. breve β-gal I (•) and β-gal II (○).

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posted on 04.08.2014 by Sheryl Lozel Arreola, Montira Intanon, Jasmina Suljic, Roman Kittl, Ngoc Hung Pham, Paul Kosma, Dietmar Haltrich, Thu-Ha Nguyen

The reaction was performed at 30°C at an initial lactose concentration of 200 g L−1 in sodium phosphate buffer (pH 6.5) and 1 mM MgCl2 using 1.5 ULac mL−1. Values are the mean of two independent experiments and the standard deviation was always less than 5%.

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