Time course of GOS formation (A) and formation and degradation of GOS during lactose conversion (B) catalyzed by <i>B. breve</i> β-gal I (•) and β-gal II (○).
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posted on 2014-08-04, 03:13 authored by Sheryl Lozel Arreola, Montira Intanon, Jasmina Suljic, Roman Kittl, Ngoc Hung Pham, Paul Kosma, Dietmar Haltrich, Thu-Ha Nguyen<p>The reaction was performed at 30°C at an initial lactose concentration of 200 g L<sup>−1</sup> in sodium phosphate buffer (pH 6.5) and 1 mM MgCl<sub>2</sub> using 1.5 U<sub>Lac</sub> mL<sup>−1</sup><sub>.</sub> Values are the mean of two independent experiments and the standard deviation was always less than 5%.</p>
