Time-course analyses in AIZ-AR cells after DHT treatment.
Cells were treated with 5α-dihydrotestosterone (DHT; 100 nM) and vehicle (DMSO; 0.1% v/v) for time periods from 2 h to 72 h. After the incubations, luciferase activity was measured in cell lysates. Data are mean of triplicate measurements and are expressed as relative luciferase units (RLU). Fold induction over DMSO-treated cells was calculated and it is shown above the bars in the graph. Similar data were obtained from two consecutive passages. Panel A: Long-term time-course analyses; incubation times: 24 h, 48 h and 72 h. Panel B: Short-term time-course analyses; incubation times: 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, 14 h, 16 h, 18 h, 20 h, 22 h and 24 h.