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Thyroid hormone mediated ERK activation stimulates PIMT phosphorylation and enhances hepatic gluconeogenesis in rats.

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posted on 2013-12-17, 03:30 authored by Bandish Kapadia, Navin Viswakarma, Kishore V. L. Parsa, Vasundhara Kain, Soma Behera, Sashidhara Kaimal Suraj, Phanithi Prakash Babu, Anand Kar, Sunanda Panda, Yi-jun Zhu, Yuzhi Jia, Bayar Thimmapaya, Janardan K. Reddy, Parimal Misra

Rats (n=5) were injected intraperitoneally (0.1mL/animal) with L-thyroxine solution for 2 weeks. Post treatment animals were sacrificed, serum was collected for detecting levels of T3 (A) and T4 (B) and whole body weight (C) were measured. (D) PIMT immunoprecipitated lysates from liver tissue were analyzed by western blotting with phospho-ERK1/2 and total ERK1/2 antibodies. (E)PIMT was immunoprecipitated from liver tissue lysates (Set1: 3 control and 3 treated; Set2: 2 control and 2 treated) separated on 10% SDS PAGE and probed with Anti-MPM2 followed by Anti-PIMT as mentioned. (F) Chromatin immunoprecipitation assay was performed in liver tissue lysates (one control and one treated in both Set1 and Set2) using Anti-PIMT or mock Anti-goat IgG on rat PEPCK promoter. (G) The liver homogenates were analyzed using Anti-PEPCK (top panel) or Anti beta actin (bottom panel). (H) Overnight fasting blood sugar was measured using glucose strip.