The relationship between individual levels of released phosphorylated-HSP27 and the levels of PDGF-AB induced by collagen in type 2 DM patients.
The level of phosphorylated-HSP27 and PDGF-AB in the supernatant of the conditioned mixture after platelet aggregation stimulated by 0.3 μg of collagen for 30 min was determined using specific ELISA kits. The baseline levels in unstimulated samples were subtracted from each of the individual released phosphorylated-HSP27 levels and the PDGF-AB levels. Each data were collected with the platelet counts, and were plotted and analyzed by linear regression analysis. (a) Whole subjects (n = 35) were plotted. (b) The residual subjects after excluding what concentration of phosphorylated-HSP27 could not be detected (n = 30) were plotted.