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The numerical dominance of inflationary cells is only evident within the blood exposed T cell population.

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posted on 2014-07-03, 03:01 authored by Corinne J. Smith, Holly Turula, Christopher M. Snyder

Mice infected with K181 MCMV for more than 3 months were injected with fluorochrome labeled anti-CD8α antibody to identify T cells exposed to the blood supply. (A) Absolute numbers of M38- and M45-specific in the labeled fraction (left panel) and unlabeled fraction (right panel) in the indicated organs. Error bars represent the standard error of the mean. (B) The ratio of M38-specific to M45-specific CD8s was derived from numbers shown in 7A. The left panel compares this ratio in the labeled and unlabeled fractions within each organ. Each line connects the populations found in an individual mouse (n = 15). The right panel shows the same comparison between IE3-specific (inflationary) and M57-specific (non-inflationary) CD8s using numbers derived from Figure S6D. Statistical significance was measured by paired student's t-test (*p<.05**p<.01, ***p<.001, **** p<.0001). (C) A global picture of MCMV-specific CD8 distribution was determined by calculating the average number of tetramer stained cells in the labeled and unlabeled compartments of the spleen, liver, lung, cervical and mediastinal lymph nodes, kidney, female reproductive tract, salivary gland, and mammary gland (n = 7−15). (C) The left panel shows the fraction of the total tetramer positive population that is localized to each site for M38-specific (top) and M45-specific CD8s (bottom). The blood localized fractions for each organ are displayed in red and the unlabeled fractions are displayed in blue. The right panel shows the distribution of only the cells that are shielded from i.v. staining. The numbers represent the percentage of cells at each site out of the total tetramer specific population.