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The molecular mechanism of reduction of meningioma cell survival by TGM2 inhibitor.

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posted on 2014-09-23, 03:19 authored by Yin-Cheng Huang, Kuo-Chen Wei, Chen-Nen Chang, Pin-Yuan Chen, Peng-Wei Hsu, Carl P. Chen, Chin-Song Lu, Hung-Li Wang, David H. Gutmann, Tu-Hsueh Yeh

(A) Cell viability was assessed by using the MTT assay and was expressed as percentage of control. Meningioma cell survival was measured at 24- and 48-hour after application of cystamine in 1, 10, 100, 1000 µM concentration. Meningioma cell viability was reduced to 39.6% of control 48 hours after cystamine treatment. (B) Western blotting for cleaved caspase-3 and p-Akt. Quantification of immunoblotting assay showed that cystamine resulted in (C) a 1.5-fold increase in caspase-3 activation relative to controls and (D) a 30% reduction in Akt phosphorylation (quantification of data from 4 independent experiments).