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The location of the BBE binding site using unfolding mechanism of BSA and site specific marker.

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posted on 19.02.2013, 17:32 by Nayana Kamtekar, Anita Pandey, Neeraj Agrawal, Raghuvir R. S. Pissurlenkar, Mohanish Borana, Basir Ahmad

Stern-Volmer plots of BBE-induced fluorescence quenching of BSA with increasing concentration of urea (A). The inset shows the effect of increasing concentrations of urea on the BBE-induced fluorescence quenching of the protein at a fixed BBE/BSA molar ratio of 2∶1. Site specific marker displacement experiments.Stern-Volmer plots of BBE-induced fluorescence quenching of BSA pre-incubated with different concentrations of diazepam, a subdomain IIIA site marker (B).

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