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The lack of hordeins in extracts of the hordein double-null line ULG 2.0.

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posted on 01.03.2013, 10:55 by Gregory J. Tanner, Malcolm J. Blundell, Michelle L. Colgrave, Crispin A. Howitt

Total hordeins (2 µg) from ULG 2.0 were subject to 1D SDS-PAGE, blotted and visualised as above and compared to standard proteins (M; Benchmark Protein Ladder, Invitrogen). The location of known proteins was confirmed by protein sequencing of replicate gels [33]: 6, γ-3-hordein; 7, β-glucosidase; 8, LTP-1.