The interaction between Dvl1 and Eps8 is required for Wnt3a-mediated axonal remodeling.
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(A) Dvl1ΔPDZ does not affect the localisation of Eps8 in N2a cells. (B) Expression of the PDZ domain of Dvl1 in N2a cells decreases the binding of Dvl1 and Eps8 by 38%, as assessed by co-immunoprecipitation experiments. (C) DRG neurons expressing the PDZ domain of Dvl1 were treated with Wnt3a for 2 hrs. Scale bar: 5μm. (D) In GFP-expressing cells Wnt3a increases the percentage of cells that show axonal remodelling (% of cells remodelled). In contrast, neurons expressing the PDZ domain of Dvl1 do not remodel in the presence of Wnt3a. (E) Proposed model for the role of Eps8 in Wnt3a-mediated axonal remodelling. Wnt3/3a induces the formation of highly dynamic actin filaments and growth cone enlargement. These effects are mediated though the direct interaction of Dvl1 and Eps8, and Gsk3β inhibition possibly induced by Eps8-Akt signalling. *p<0.05.