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The induction of apoptosis by IPA-3.

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posted on 19.07.2013, 01:43 by Leo Lap-Yan Wong, Ian Pak-Yan Lam, Tracy Yuk-Nar Wong, Wai-Lung Lai, Heong-Fai Liu, Lam-Lung Yeung, Yick-Pang Ching

(A) Annexin V-7ADD staining assay. The fluorescence signals of annexin V-PE and 7-AAD were detected with PE-A and Per-Cy5-5-A channels, respectively (upper panel). The percentage of H2M cells stained with annexin V-PE only under different treatments was shown in a bar chart (lower panel). *P<0.001 (ANOVA) compared with the DMSO control. (B) Serum-starved H2M cells were treated with increasing dosages of IPA-3 together with serum replenishment for 24 hours. Western blotting analysis of PARP1, P-PAK1 (T423), total PAK1 and cleaved caspase 3 was performed.


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