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The expression of the reporter gene in NDL-PCB (non-dioxin-like polychlorinated biphenyl) Reporters was insensitive to endogenous secondary metabolites in living cells.

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posted on 13.11.2013, 02:43 authored by Lieming Bao, Chen Gao, Miaomiao Li, Yong Chen, Weiqiang Lin, Yanjun Yang, Ning Han, Hongwu Bian, Muyuan Zhu, Junhui Wang

(a) Histochemical assay of GUS activity in young seedlings of NDL-PCB Reporters. Three-day-old seedlings were transferred to plates without (control) or with 50 nM PCB 18 (+ PCB 18) and grown for 3 days. The right root under PCB 18 treatment was stained blue. (b) Histochemical assay of GUS activity in 12-day-old NDL-PCB Reporters. Six-day-old seedlings were transferred to plates with 50 nM PCB 28 and grown for 6 days. Roots under PCB 28 treatment were stained blue. (c) NDL-PCB Reporters were insensitive to endogenous auxin in root tips as no GFP signals were observed by confocal microscopy. The red PI (propidium iodide) indicated the cell wall. (d) The DR5::GFP line reported auxin peaks (GFP signals) in roots. (e) Naringenin chalcone (yellow fluorescence) in distal elongation zones and meristems of 10-d-old Arabidopsis roots. Meristematic cells were small in size. (f) Kaempferol and quercetin (gold fluorescence) in mature elongation zones of 10-day-old Arabidopsis roots. Bars for (c) to (f), 30 µm.