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The expression of the N-PmpC was quantified using Western blot.

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posted on 2015-12-17, 08:07 authored by Aleksandra Inic-Kanada, Marijana Stojanovic, Simone Schlacher, Elisabeth Stein, Sandra Belij-Rammerstorfer, Emilija Marinkovic, Ivana Lukic, Jacqueline Montanaro, Nadine Schuerer, Nora Bintner, Vesna Kovacevic-Jovanovic, Ognjen Krnjaja, Ulrike Beate Mayr, Werner Lubitz, Talin Barisani-Asenbauer

BGs expressing major outer membrane protein (MOMP) were used as standards. Line 1, molecular weight markers; line 2, N-PmpC expressed within EcN BGs (MW 92.1 kD), line 3, 560ng MOMP standard 1; line 4, 760ng MOMP standard 2; line 5, 1200ng MOMP standard 3; line 6, 1600ng MOMP standard 4. The membrane was developed using anti-myc-HRP antibody. Quantification of the N-PmpC was performed with the QuantityOne Software in the ChemiDocXRS program.