The expression of PLKs in THP-1 cells.

(A) Quantitative expression of the PLK genes. THP-1 cells were cultured for 24 h in 6-well plates. The gene expression of PLKs was detected by qRT-PCR. PLK1 gene expression was designated as 100%. PLK2-4 gene expression was shown as percentage expression compared with PLK1 gene expression. (B) The effect of GW843682X on the protein expression of the PLKs. THP-1 cells were treated with 10 µM GW843682X for the indicated time periods. The protein expression of PLKs was detected by western blot. β-actin protein was detected as a loading control. (C) The effect of GW843682X on the gene expression of the PLKs. THP-1 cells were treated with 10 µM GW843682X for the indicated time periods. The gene expression of the PLKs was detected by qRT-PCR. Gene expression in the GW843682X-treated groups was shown as the fold expression relative to the non-treated groups.