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The expression of MMP-7 within atherosclerotic carotid plaques in relation to plaque symptomatology.

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posted on 2014-01-06, 02:50 authored by Azhar Abbas, Pål Aukrust, David Russell, Kirsten Krohg-Sørensen, Trine Almås, Dorte Bundgaard, Vigdis Bjerkeli, Ellen Lund Sagen, Annika E. Michelsen, Tuva B. Dahl, Sverre Holm, Thor Ueland, Mona Skjelland, Bente Halvorsen

mRNA (A) and protein (B) levels of MMP-7 in atherosclerotic carotid plaques were measured in (i) patients with the most recent symptoms (within the last 2 months, n = 35) and (ii) patients with symptoms >2 months ago and asymptomatic patients (>2 months, n = 29). For comparison, mRNA levels of MMP-7 were also analyzed in non-atherosclerotic vessels (common iliac artery) from 10 organ donors (CTR). mRNA levels were quantified by real-time RT-PCR. The expression of β-actin was used as endogenous control. Protein levels of MMP-7 were measured in plaque lysates by EIA. Data are shown as a box and whisker plot with median (Q1, Q3) in the box and the whiskers representing the 5 and 95 percentile in relation to β -actin expression.