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The effects of PMBPs on apoptosis-related proteins.

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posted on 05.11.2015 by Jia Liu, Jing Bai, Guoqiang Jiang, Xinli Li, Jing Wang, Dachang Wu, Lawrence Owusu, Ershao Zhang, Weiling Li

(A,B) The A2780 and OV2008 cells were treated with PMBPs (0, 15, 25, 35, 50 μg/ml) for 24 h and then harvested. Cell lysates were prepared and subjected to Western blot analysis. The protein levels of Bcl-2, cleaved-caspase-3 and cleaved-caspase-9 were measured by Western blot. (C,D,E) Histograms show mean expression levels of Bcl-2, cleaved-caspase-3 and cleaved-caspase-9 (±SD), respectively, from three independent experiments. Bcl-2, cleaved-caspase-3 and cleaved-caspase-9 levels were expressed relative to loading control, β-Actin. All experiments were repeated three times.**P<0.01 compared to the control group.

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