The effects of AhR knock-down and over-expression on αB-crystallin expression.
A. 80 pmol of siRNA targeting AhR mRNA (si-AhR) or non-targeting siRNA (si-ctrl) were transfected into αTN4 cells and total cell proteins were extracted 60 h later. Expression of AhR and αB-crystallin were determined by Western immunoblotting. B. 0.1, 0.25, 0.5 and 1 µg pcDNA3.1/B6AhR and pcDNA/ARNT were co-transfected into αTN4 cells and total cell proteins were extracted 24 h later. AhR, ARNT and αB-crystallin were detected by Western immunoblotting. β-actin was detected as an internal control. ( *, p<0.05; **, p<0.01; ***, p<0.001).