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The effects of ABA on endoreduplication.

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posted on 19.02.2015, 02:43 by Jan F. Humplík, Véronique Bergougnoux, Michaela Jandová, Jan Šimura, Aleš Pěnčík, Ondřej Tomanec, Jakub Rolčík, Ondřej Novák, Martin Fellner

The influence of ABA on nuclear ploidy was investigated using hypocotyl segments of sit and WT seedlings grown in darkness on untreated media and media containing 100 nM ABA (A). Triangles represent all measured values (n = 40) for each genotype and treatment. The bars within the boxes indicate the median values in each case, while the boxes’ upper and lower boundaries indicate the boundaries of the first and third quartiles. Three independent experiments were performed. The Kruskal-Wallis ANOVA test with multiple comparisons revealed that only the sit control sample differed significantly from the WT control (p < 0.01). No other significant differences were found. (B) Analysis of the expression of the SlKRP1 and SlKRP3 genes based on the mean of three independent experiments ± SE. Tip41like and PP2ACs were used as housekeeping genes. All expression values are quoted relative to those for the “WT control” sample.