The effect of recombinant NAMPT protein on OGD-induced cell necrosis in neuron-glial mixed culture.

Wild type NAMPT (WT) or H247A NAMPT (H247A) protein was applied 30 min before OGD. (A and B) Representative images of triple immunofluorescence staining of NeuN (green, neuron specific marker), PI (red, for necrotic cells) and DAPI (blue, for all cells) under normal condition (A) or after OGD and recovery (B). (C) Percentage of necrotic cells in total cells (PI-positive cell number over DAPI-positive number). N=6. (D) Percentage of necrotic neurons in total neuron cells (PI/NeuN double positive cell number over NeuN-positive cell number). N=6. *P<0.05, **P<0.01, ***P<0.001, compared with the control of OGD, One-way ANOVA. ^###P<0.001, compared with the control of control, One-way ANOVA.