The effect of a high-fat diet (HFD) on the leukocyte populations visceral adipose tissues.
Number of leukocyte subtypes per mm2 of gonadal visceral adipose tissue (A) and mesenteric adipose tissue (B) in control female (n = 4 and 6, respectively), HFD female (n = 7 and 6), control male (n = 5 and 6) and HFD male (n = 8 and 6) mice. F4/80+MGL-1+ are resident macrophages (M2-like) and F4/80+MGL-1- are inflammatory macrophages (M1-like). P<0.05 for the F4/80+MGL-1− macrophages compared with control (*), compared with female control (†) and compared with female on HFD (‡). C. Representative images of stained macrophages in gonadal adipose tissue of control female, HFD female, control male, and HFD male mice. M2-like macrophages are double positive for MGL-1 (Alexa Fluor488, green) and F4/80 (NL557, red), while M1-like macrophages are only positive for F4/80 (red). Nuclei are stained blue (Hoechst33342). Scale bar equals 50 µm and arrows mark crown-like structures. D. Foxp3 mRNA levels in gonadal adipose tissue (n = 6 in all groups). Foxp3 Ct-values were normalized to 18S and the mean of the linearized expression values from female mice on control diet were set as 100%. E. Percentage of Foxp3-positive cells in gonadal and mesenteric adipose tissue in control female (n = 10), HFD female (n = 10), control male (n = 12), and HFD male (n = 13) mice after 14 weeks on HFD. All data are means ± SEM. P<0.05 compared with control (*), compared with female control (†) and compared with female on HFD (‡).